SCREENING OF ANALGESIC ACTIVITY OF CHLOROFORM EXTRACT OF SOLANUM NIGRUM IN SWISS ALBINO MICE

Abstract

Background: Pain is the one of the major symptom in any pathological condition. Various drugs are available to reduce the pain but they are associated with various adverse drug reactions. In Ayurveda Solanmum nigrum leaves are used to reduce the pain. The present study aimed to screen the analgesic activity of Solanmum nigram leaves in Swiss Albino mice.

Materials and Methods: The study was conducted in the Department of Pharmacology, Sree Mookambika Medical College, Tamil Nadu. Total of 24 mice were divided into 4 groups each of 6 rats. G-I (0.9% normal saline), G-II (Morphine 5 mg/kg), G-III (Solanmum nigram 100mg/kg) and G-IV (Solanmum nigram 200 mg/kg). The analgesic activity was evaluated by tail clip and tail flick methods. In both methods reaction time was noted after 30min of drug administration. Mean reaction time was calculated and compared between the groups by using SPSS (16.0 version) soft ware.

Results: The results of tail clip and flick methods are group-I showed significant difference compared group-II, III and IV. Group-II showed significant difference with group-III but not with group-IV. Group-III showed significant difference compared to group-IV. In both methods reaction time was increased compared to group-II with IV but not statistically significant.

Conclusion: High dose of chloroform extract of Solanmum nigrum showed significant analgesic activity compared to its low dose. It can be used as analgesic where synthetic drug are contraindicated.

Keywords: Analgesic activity, chloroform, Solanum Nigrum, tail clip, tail flick, reaction time