SCREENING OF ANTI-RHEUMATOID EFFECT OF AQUEOUS EXTRACT OF SESAMUM INDICUM SEEDS IN FREUND’S COMPLETE ADJUVANT INDUCED ARTHRITIS IN WISTAR ALBINO RATS

Abstract

Background: Rheumatoid arthritis (RA) is autoimmune disorder. It affects the all parts of the body. The present study aimed to screen the anti-rheumatoid effect of aqueous extract of Sesamum indicum seeds in Freund’s complete adjuvant induced arthritis in Wistar Albino rats

Materials and Methods: Wistar Albino male rats weighing 180-200gm were used in this study. A total of 30 rats were divided into 5 groups each of 6 rats. G-I (Normal Saline 0.9%), G-II Freund’s adjuvant (0.1 ml/0.5%), G-III Dexamethasone (0.5mg/kg), G-IV Aqueous extract of Sesamum indicum (400mg/kg) and G-V  Aqueous extract of Sesamum indicum (800mg/kg). 28 days drugs were given to their respective groups. On 1^st, 7^th, 14^th and 28^th day blood samples were collected and serum was separated. The collected serum was used for the analysis of rheumatoid factor, C-reactive protein and erythrocyte sedimentation rate. Statistical Package for Social Sciences (SPSS 16.0) version used for statistical analysis.

Results:                The study results showed that group-I showed significant effect compared to all other groups at 4 time periods. Group-II showed significant increase all the parameters compared to other groups. Group-III and V showed significant decrease in all the parameters compared to group-II. Group-IV showed significant effect with compared to group-I, II, III and V. Comparison between group-III and V not showed any significant effect.

Conclusion: High dose of aqueous extract of Sesamum indicum seeds showed significant decrease in ESR, CRP and RAF. The results are similar to standard drug.

Keywords: Aqueous extract, C-reactive protein, Dexamethasone, Rheumatoid arthritis, Rheumatoid factor, Sesamum indicum seeds